Biochemical effects of Pleurotus tuberregium on lipid peroxidation and the activities of superoxide dismutase and catalase
Abstract
This work investigated the probable protective effects of an aqueous extract of Pleurotus tuberregium on cadmium-induced oxidative stress and activities of some antioxidant enzymes in the heart and testes of male albino rats.
Method: Male albino rats were assigned into four groups of ten animals each in this study that lasted for 4 weeks. The control group was administered normal saline whereas the other groups were all orally treated with 3 mg/kg body weight cadmium chloride and two of the test groups were also gavage-fed different doses of aqueous extract of Pleurotus tuberregium (either 500 or 1 000 mg/kg body weight). Lipid peroxidation and the activities of superoxide dismutase (SOD) and catalase (CAT) as well as the concentration of cadmium were determined in the heart and testes of the animals.
Results: The concentration of cadmium in the heart and testes of the rats that were not treated with the aqueous extract of Pleurotus tuberregium were significantly (p<0.05) higher when compared with the controls. However, administration of Pleurotus tuberregium appeared to reduce the level of cadmium in these organs towards values comparable with the controls.
The concentration of malondialdehyde also exhibited the same trend. The activities of SOD and CAT were significantly (p<0.05) decreased in the rats treated with cadmium compared with the controls. However, administration of the aqueous extract of Pleurotus tuberregium seemed to ameliorate the effect of cadmium on the activities of SOD and CAT as there was no significant difference (p>0.05) when compared with the controls.
Conclusion: The results of this study suggest that the aqueous extract of Pleurotus tuberregium may be able to considerably reduce the oxidative stress engendered by cadmium in rats.
Method: Male albino rats were assigned into four groups of ten animals each in this study that lasted for 4 weeks. The control group was administered normal saline whereas the other groups were all orally treated with 3 mg/kg body weight cadmium chloride and two of the test groups were also gavage-fed different doses of aqueous extract of Pleurotus tuberregium (either 500 or 1 000 mg/kg body weight). Lipid peroxidation and the activities of superoxide dismutase (SOD) and catalase (CAT) as well as the concentration of cadmium were determined in the heart and testes of the animals.
Results: The concentration of cadmium in the heart and testes of the rats that were not treated with the aqueous extract of Pleurotus tuberregium were significantly (p<0.05) higher when compared with the controls. However, administration of Pleurotus tuberregium appeared to reduce the level of cadmium in these organs towards values comparable with the controls.
The concentration of malondialdehyde also exhibited the same trend. The activities of SOD and CAT were significantly (p<0.05) decreased in the rats treated with cadmium compared with the controls. However, administration of the aqueous extract of Pleurotus tuberregium seemed to ameliorate the effect of cadmium on the activities of SOD and CAT as there was no significant difference (p>0.05) when compared with the controls.
Conclusion: The results of this study suggest that the aqueous extract of Pleurotus tuberregium may be able to considerably reduce the oxidative stress engendered by cadmium in rats.
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