Phytochemical and in vitro antibacterial activity of crude extracts of the seeds of Parinari polyandra (Rosaceae)
Abstract
Phytochemical and antibacterial properties of aqueous and methanolic extracts of the Parinari polyandra Seed (Rosaceae) used in herbal medicine by Traditional Medicine Practitioners (TMPs) in Ilorin; Kwara State were screened and
tested against five pathogenic bacterial.
Phytochemical screening of the crude extracts of the seeds Parinari polyandra showed the presence of tannin, saponin, cardiac glycoside, terpenoids, flavoniods and volatile oil. The in-vitro antibacterial properties of aqueous and methanolic
extracts of the plants was determined by agar diffusion technique, using pure isolates of Staphylococcus aureus (ATCC 259230), Esherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Proteus specie. The results of the zone of inhibition showed that the aqueous and methanolic extracts of Parinari polyandra have antibacterial activity (25.33 ± 1.24mm and 29.67± 1.20 mm respectively) on standard Staphylococcus aureus (ATCC 259230); while methanolic extract of Parinari polyandra showed bioactivity on Esherichia coli (18.00 ± 0.94 mm) at concentration of 15mg/ml.
Comparatively, methanolic extract of the seeds of Parinari polyandra has a significant higher in-vitro antibacterial activity than the aqueous extract and compared favourably with Ampicillin trihydrate control. The minimum inhibitory concentrations (MIC) of the aqueous and methanolic extracts of the seeds of Parinari polyandra on standard Staphylococcus aureus (ATCC 259230) are 40mg/ml and 25mg/ml respectively. The crude aqueous and methanolic extracts of these plants possess antibacterial properties to justify their traditional use in herbal medicine.
tested against five pathogenic bacterial.
Phytochemical screening of the crude extracts of the seeds Parinari polyandra showed the presence of tannin, saponin, cardiac glycoside, terpenoids, flavoniods and volatile oil. The in-vitro antibacterial properties of aqueous and methanolic
extracts of the plants was determined by agar diffusion technique, using pure isolates of Staphylococcus aureus (ATCC 259230), Esherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Proteus specie. The results of the zone of inhibition showed that the aqueous and methanolic extracts of Parinari polyandra have antibacterial activity (25.33 ± 1.24mm and 29.67± 1.20 mm respectively) on standard Staphylococcus aureus (ATCC 259230); while methanolic extract of Parinari polyandra showed bioactivity on Esherichia coli (18.00 ± 0.94 mm) at concentration of 15mg/ml.
Comparatively, methanolic extract of the seeds of Parinari polyandra has a significant higher in-vitro antibacterial activity than the aqueous extract and compared favourably with Ampicillin trihydrate control. The minimum inhibitory concentrations (MIC) of the aqueous and methanolic extracts of the seeds of Parinari polyandra on standard Staphylococcus aureus (ATCC 259230) are 40mg/ml and 25mg/ml respectively. The crude aqueous and methanolic extracts of these plants possess antibacterial properties to justify their traditional use in herbal medicine.
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