Overproduction of ammonia by a local isolate of Staphylococcus aureus in a cheaply fabricated medium
Abstract
Upon screening a number of formulated media for ammonia overproduction by Staphylococcus aureus,
an ammonia concentration of 13.09 mg/ml was obtained. the medium used was 10% concentration of fish hydrolysate
prepared by 5% HCl digestion. Ammonia yield on synthetic peptone broth was 12.75 mg/ml. Physical parameters such
as incubation time, temperature, effect of aeration and agitation were investigated for an optimization of the bioprocess.
Key Words: Ammonia; Overproduction; Metabolites; Formulated peptone; Staphylococcus aureus
an ammonia concentration of 13.09 mg/ml was obtained. the medium used was 10% concentration of fish hydrolysate
prepared by 5% HCl digestion. Ammonia yield on synthetic peptone broth was 12.75 mg/ml. Physical parameters such
as incubation time, temperature, effect of aeration and agitation were investigated for an optimization of the bioprocess.
Key Words: Ammonia; Overproduction; Metabolites; Formulated peptone; Staphylococcus aureus
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