The Hepato-Renal Effect of Graded Doses of a Glyphosate-Based Herbicide (Roundup) in Rat
Abstract
Roundup, one of the most widely used glyphosate-based herbicides is classified as hazardous to the environment. Its low persistence leads to repeated applications and consequently, large portions contaminate
soil and water bodies, causing harm to non-target species. The hepato-renal toxicity of graded doses (300, 400 and 600 mgKg-1body wt) of roundup on selected plasma metabolites, enzymes and hematological parameters
was investigated in rats (Wistar strain; 207g± 6 g). Thirty–two male rats were divided into four groups; control (n = 8) and test groups (n = 24), divided into 3 groups of eight rats each that received graded doses of the herbicide, respectively for 8 days. Hepatotoxicity was investigated by quantitative analysis of plasma alanine transaminase (ALT), alkaline phosphatase (ALP), total protein, albumin, globulin and cholesterol levels. Renal-toxicity was determined by quantitative analysis of serum creatinine, urea, K+ and Na+ levels. Relative to the control, there was significant (p<0.05) reduction in the activities of ALT and ALP while plasma total protein, albumin and globulin levels were significantly (p<0.05) elevated in the groups that received high doses of the herbicide. Creatinine, Urea, Na+ and hematocrit were significantly (p<0.05) elevated while K+, White Blood Cell and Lymphocyte were significantly (p<0.05) reduced in the groups that received the high doses of the herbicide, relative to the control. Histopathological studies revealed periportal steatosis and mild necrosis in the liver and thickened alveolar septae filled with inflammatory cells in the lung tissues. This study shows that roundup is toxic to the hepatorenal tissues of rat.
soil and water bodies, causing harm to non-target species. The hepato-renal toxicity of graded doses (300, 400 and 600 mgKg-1body wt) of roundup on selected plasma metabolites, enzymes and hematological parameters
was investigated in rats (Wistar strain; 207g± 6 g). Thirty–two male rats were divided into four groups; control (n = 8) and test groups (n = 24), divided into 3 groups of eight rats each that received graded doses of the herbicide, respectively for 8 days. Hepatotoxicity was investigated by quantitative analysis of plasma alanine transaminase (ALT), alkaline phosphatase (ALP), total protein, albumin, globulin and cholesterol levels. Renal-toxicity was determined by quantitative analysis of serum creatinine, urea, K+ and Na+ levels. Relative to the control, there was significant (p<0.05) reduction in the activities of ALT and ALP while plasma total protein, albumin and globulin levels were significantly (p<0.05) elevated in the groups that received high doses of the herbicide. Creatinine, Urea, Na+ and hematocrit were significantly (p<0.05) elevated while K+, White Blood Cell and Lymphocyte were significantly (p<0.05) reduced in the groups that received the high doses of the herbicide, relative to the control. Histopathological studies revealed periportal steatosis and mild necrosis in the liver and thickened alveolar septae filled with inflammatory cells in the lung tissues. This study shows that roundup is toxic to the hepatorenal tissues of rat.
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