Effects of Different Crude Oil Fractions on Total Chlorophyll, Glucose and Starch Content of Maize (Zea mays)
Abstract
This study was carried out to determine the effects of different crude oil fractions on total chlorophyll content, glucose and sugar content of Zea mays. The maize seeds were planted in polythene bags in the Department of
Biochemistry, Faculty of Life Sciences Laboratory garden. A total of 300 bags, each containing 500g of sandy loam soil, with no known history of crude oil contamination were used for this study. 270 bags were used for planting test samples of 2%, 5% 10% crude oil fraction while 30 bags served as control for planting maize. The experiment lasted for a period of about one month. The seedlings were harvested after 7, 14, and 21 days post germination and the biochemical analysis of the roots was determined in the Department of Biochemistry and School of Basic Medical Sciences in University of Benin City, Nigeria. The data obtained were subjected to descriptive statistic of variance analysis. Crude oil at all levels of contamination and for all fractions on soil, significantly reduced total
chlorophyll content in the leaves and starch contents in the roots of maize seedlings. In contrast, the glucose content significantly increased in the roots for all fractions at all levels of contamination of the soil. The significance of the increasing glucose content in the maize plant as a result of crude oil contamination is discussed.
Biochemistry, Faculty of Life Sciences Laboratory garden. A total of 300 bags, each containing 500g of sandy loam soil, with no known history of crude oil contamination were used for this study. 270 bags were used for planting test samples of 2%, 5% 10% crude oil fraction while 30 bags served as control for planting maize. The experiment lasted for a period of about one month. The seedlings were harvested after 7, 14, and 21 days post germination and the biochemical analysis of the roots was determined in the Department of Biochemistry and School of Basic Medical Sciences in University of Benin City, Nigeria. The data obtained were subjected to descriptive statistic of variance analysis. Crude oil at all levels of contamination and for all fractions on soil, significantly reduced total
chlorophyll content in the leaves and starch contents in the roots of maize seedlings. In contrast, the glucose content significantly increased in the roots for all fractions at all levels of contamination of the soil. The significance of the increasing glucose content in the maize plant as a result of crude oil contamination is discussed.
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