In Vitro Studies of Antiproliferative Properties of Aqueous leaf Extracts of Moringaoleifera and Globimetulabrauniion on the Human Pancreatic Cell Line AsPC-1
Abstract
Cancer of the pancreas is the fourth deadliest form of cancer in the world as symptoms do not manifest until the advanced stages. Conventional methods of treatment have less than 10% rate of success. Therefore the need exists
to develop anticancer agents from medicinal plants to support conventional methods. The objective of this study was therefore to determine the anti-proliferative effect of aqueous Moringa oleifera and Globimetula braunii leaf extracts on the human pancreatic cancer AsPC-1 cell line. Human pancreatic (AsPC-1) cells were seeded (1.07 x10 4 cells/well) into 96 well plates and exposed to graded (0 – 10000 μg mlˉ1) concentrations of each extract for 24 hours, thereafter the anti-proliferative effect of the extracts were evaluated in -vitro using the MTT [3-(4,5- dimethylthiazol–2-yl)-2,5-diphenyltetrazolium bromide] colorimetric assay. Leaf extracts of Moringa oleifera and Globimetula braunii exhibited dose dependent inhibition of AsPC-1 cell proliferation, with IC 50 values of 156.25 μg mlˉ1 and 312.50 μg mlˉ1, respectively. Of both plant extracts tested on AsPC-1, the aqueous leaf extract of had a higher inhibitory effect. This result suggests the presence of key phytochemical or a group of phytochemicalcompounds with anti-proliferative properties such as flavonoids, tannins or cyanogenic glycosides in the testedextracts.
to develop anticancer agents from medicinal plants to support conventional methods. The objective of this study was therefore to determine the anti-proliferative effect of aqueous Moringa oleifera and Globimetula braunii leaf extracts on the human pancreatic cancer AsPC-1 cell line. Human pancreatic (AsPC-1) cells were seeded (1.07 x10 4 cells/well) into 96 well plates and exposed to graded (0 – 10000 μg mlˉ1) concentrations of each extract for 24 hours, thereafter the anti-proliferative effect of the extracts were evaluated in -vitro using the MTT [3-(4,5- dimethylthiazol–2-yl)-2,5-diphenyltetrazolium bromide] colorimetric assay. Leaf extracts of Moringa oleifera and Globimetula braunii exhibited dose dependent inhibition of AsPC-1 cell proliferation, with IC 50 values of 156.25 μg mlˉ1 and 312.50 μg mlˉ1, respectively. Of both plant extracts tested on AsPC-1, the aqueous leaf extract of had a higher inhibitory effect. This result suggests the presence of key phytochemical or a group of phytochemicalcompounds with anti-proliferative properties such as flavonoids, tannins or cyanogenic glycosides in the testedextracts.
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